Effectiveness of an indigenous Bacillus subtilis B6 strain in the control of postharvest apple fruit rot
Само за регистроване кориснике
2023
Аутори
Hrustić, JovanaMihajlović, Milica
Stevanović, Marija
Gašić, Slavica
Grahovac, Mila
Tanović, Brankica
Остала ауторства
van den Bosch, FrankЧланак у часопису (Објављена верзија)
,
Koninklijke Nederlandse Planteziektenkundige Vereniging
Метаподаци
Приказ свих података о документуАпстракт
Antifungal activity of 108 Bacillus spp. isolates, originating from the rhizosphere of different
cultivated plants, against several causal agents of apple fruit rot (Monilinia fructicola, Colletotrichum acutatum and Botrytis cinerea) were studied in vitro. Based on the results of dual culture and in vitro antifungal assays, a B6 strain was selected for further investigation. Molecular characterization of nucleotide sequences derived from PCR-amplified 16S rDNA fragments confirmed that the B6 strain belongs to the species Bacillus subtilis. In addition, PCR analysis showed that the strain genome included genes involved in the production of antimicrobial substances (iturin, bacillomycin and surfactin). Regarding the antimicrobial activity of B6 filtrates, the results indicated that competition for nutrients and antibiosis were the most probable modes of action. Based on the results obtained in in vivo experiments on apple fruits with demonstrated efficacy of 70–93%, three different liqu...id formulations were developed and their effectiveness was tested against M. fructicola. As the most efficient product, formulation 3 (SE formulation prepared with esterified rape seed and nonionic emulsifier) was chosen for further testing,
and the results showed an efficacy ranging from 60 to 80%. In conclusion, this work demonstrated that the SE bioproduct based on B. subtilis strain B6 offers a potentially suitable approach for M. fructicola management on apple fruits.
Кључне речи:
Biological control / Antimicrobial activity / Postharvest decay / M. fructicola / Formulation developmentИзвор:
European Journal of Plant Pathology, 2023, 167, 727-742Издавач:
- Berlin : Springer
Пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200214 (Институт за пестициде и заштиту животне средине, Београд) (RS-MESTD-inst-2020-200214)
Напомена:
- Supplementary material https://doi.org/10.1007/s10658-023-02719-7
Институција
Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Hrustić, Jovana AU - Mihajlović, Milica AU - Stevanović, Marija AU - Gašić, Slavica AU - Grahovac, Mila AU - Tanović, Brankica PY - 2023 UR - https://ripest.pesting.org.rs/handle/123456789/609 AB - Antifungal activity of 108 Bacillus spp. isolates, originating from the rhizosphere of different cultivated plants, against several causal agents of apple fruit rot (Monilinia fructicola, Colletotrichum acutatum and Botrytis cinerea) were studied in vitro. Based on the results of dual culture and in vitro antifungal assays, a B6 strain was selected for further investigation. Molecular characterization of nucleotide sequences derived from PCR-amplified 16S rDNA fragments confirmed that the B6 strain belongs to the species Bacillus subtilis. In addition, PCR analysis showed that the strain genome included genes involved in the production of antimicrobial substances (iturin, bacillomycin and surfactin). Regarding the antimicrobial activity of B6 filtrates, the results indicated that competition for nutrients and antibiosis were the most probable modes of action. Based on the results obtained in in vivo experiments on apple fruits with demonstrated efficacy of 70–93%, three different liquid formulations were developed and their effectiveness was tested against M. fructicola. As the most efficient product, formulation 3 (SE formulation prepared with esterified rape seed and nonionic emulsifier) was chosen for further testing, and the results showed an efficacy ranging from 60 to 80%. In conclusion, this work demonstrated that the SE bioproduct based on B. subtilis strain B6 offers a potentially suitable approach for M. fructicola management on apple fruits. PB - Berlin : Springer T2 - European Journal of Plant Pathology T1 - Effectiveness of an indigenous Bacillus subtilis B6 strain in the control of postharvest apple fruit rot EP - 742 SP - 727 VL - 167 DO - 10.1007/s10658-023-02719-7 ER -
@article{ author = "Hrustić, Jovana and Mihajlović, Milica and Stevanović, Marija and Gašić, Slavica and Grahovac, Mila and Tanović, Brankica", year = "2023", abstract = "Antifungal activity of 108 Bacillus spp. isolates, originating from the rhizosphere of different cultivated plants, against several causal agents of apple fruit rot (Monilinia fructicola, Colletotrichum acutatum and Botrytis cinerea) were studied in vitro. Based on the results of dual culture and in vitro antifungal assays, a B6 strain was selected for further investigation. Molecular characterization of nucleotide sequences derived from PCR-amplified 16S rDNA fragments confirmed that the B6 strain belongs to the species Bacillus subtilis. In addition, PCR analysis showed that the strain genome included genes involved in the production of antimicrobial substances (iturin, bacillomycin and surfactin). Regarding the antimicrobial activity of B6 filtrates, the results indicated that competition for nutrients and antibiosis were the most probable modes of action. Based on the results obtained in in vivo experiments on apple fruits with demonstrated efficacy of 70–93%, three different liquid formulations were developed and their effectiveness was tested against M. fructicola. As the most efficient product, formulation 3 (SE formulation prepared with esterified rape seed and nonionic emulsifier) was chosen for further testing, and the results showed an efficacy ranging from 60 to 80%. In conclusion, this work demonstrated that the SE bioproduct based on B. subtilis strain B6 offers a potentially suitable approach for M. fructicola management on apple fruits.", publisher = "Berlin : Springer", journal = "European Journal of Plant Pathology", title = "Effectiveness of an indigenous Bacillus subtilis B6 strain in the control of postharvest apple fruit rot", pages = "742-727", volume = "167", doi = "10.1007/s10658-023-02719-7" }
Hrustić, J., Mihajlović, M., Stevanović, M., Gašić, S., Grahovac, M.,& Tanović, B.. (2023). Effectiveness of an indigenous Bacillus subtilis B6 strain in the control of postharvest apple fruit rot. in European Journal of Plant Pathology Berlin : Springer., 167, 727-742. https://doi.org/10.1007/s10658-023-02719-7
Hrustić J, Mihajlović M, Stevanović M, Gašić S, Grahovac M, Tanović B. Effectiveness of an indigenous Bacillus subtilis B6 strain in the control of postharvest apple fruit rot. in European Journal of Plant Pathology. 2023;167:727-742. doi:10.1007/s10658-023-02719-7 .
Hrustić, Jovana, Mihajlović, Milica, Stevanović, Marija, Gašić, Slavica, Grahovac, Mila, Tanović, Brankica, "Effectiveness of an indigenous Bacillus subtilis B6 strain in the control of postharvest apple fruit rot" in European Journal of Plant Pathology, 167 (2023):727-742, https://doi.org/10.1007/s10658-023-02719-7 . .