Antifungal activity of peppermint and spearmint essential oils against Trichoderma spp. green mold disease agents of oyster mushroom and shiitake
2024
Autori
Potočnik, IvanaLuković, Jelena
Stepanović, Miloš
Todorović, Biljana
Đorđević, Tijana
Đurović-Pejčev, Rada
Milijašević-Marčić, Svetlana
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Production of oyster mushroom (Pleurotus spp.) and shiitake (Lentinula edodes) is seriously affected by various
Trichoderma species causing green mold disease. The purpose of the study was to determine the antifungal activity
of essential oils peppermint (Mentha piperita) and spearmint (Mentha spicata), both obtained from plants from
Serbia, against Trichoderma strains isolated from Pleurotus ostreatus oyster mushroom (Trichoderma pleuroti KM11
and Trichoderma pleuroticola KM12, 2018, North Macedonia) and shiitake substrates (Trichoderma guizhouense
T59, 2018, Serbia). Air-dried plant material was subjected to hydro-distillation in a Clevenger type apparatus. The
obtained essential oils were dried over anhydrous sodium sulphate. Antifungal activity of the oils was tested using
in vitro methods: contact microdilution by using a pathogen spore suspension, and fumigant macrodilution by
applying either pathogen mycelial inoculum or spore suspension. Both oils were lethal for all te...sted species when
the contact microdilution method was used at 12.5 μl mL-1, while no fungicidal effect of either oil was found on any
tested strain using the fumigant method. In the contact microdilution bio-assay, both oils inhibited T. guizhouense
T59 at 3.12 μl mL-1; peppermint oil more strongly suppresed T. pleuroti KM11 (3.12 μl mL-1) than T. pleuroticola KM12
(6.25 μl mL-1), while spearment oil had an opposite effect. Using fumigation, both oils inhibitied mycelial growth of
the tested species at 0.16 μl mL-1, with an exception of spearmint oil, which inhibited T. guizhouense T59 at 0.32 μl
mL-1 of air phase. Inhibition of spore germination of T. pleuroti KM11 by fumigation was detected at 0.08 (pepermint
oil) or 0.016 μl mL-1 (spearmint oil), T. pleuroticola KM12 at 0.016 μl mL-1 (both oils), and T. ghuizouense T59 at 0.32
μl mL-1 (peppermint oil) or inhibition was missing (spearmint oil). The most resistant taxon to both oils was T.
guizhoense from shiitake. Both oils showed similar contact effect, while peppermint oil exhibited a slightly stronger
fumigant activity than spearmint oil on the tested strains. Peppermint and spearmint oils could be recommended
for further in vivo investigation.
Ključne reči:
antifungal activity / peppermint / spearmint / essential oils / Trichoderma spp. / oyster mushroom / shiitakeIzvor:
20th ISMS CONGRESS Program and Abstract Book, 2024, 50-50Izdavač:
- International Society for Mushroom Science
Projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200214 (Institut za pesticide i zaštitu životne sredine, Beograd) (RS-MESTD-inst-2020-200214)
Institucija
Institut za pesticide i zaštitu životne sredineTY - CONF AU - Potočnik, Ivana AU - Luković, Jelena AU - Stepanović, Miloš AU - Todorović, Biljana AU - Đorđević, Tijana AU - Đurović-Pejčev, Rada AU - Milijašević-Marčić, Svetlana PY - 2024 UR - https://ripest.pesting.org.rs/handle/123456789/760 AB - Production of oyster mushroom (Pleurotus spp.) and shiitake (Lentinula edodes) is seriously affected by various Trichoderma species causing green mold disease. The purpose of the study was to determine the antifungal activity of essential oils peppermint (Mentha piperita) and spearmint (Mentha spicata), both obtained from plants from Serbia, against Trichoderma strains isolated from Pleurotus ostreatus oyster mushroom (Trichoderma pleuroti KM11 and Trichoderma pleuroticola KM12, 2018, North Macedonia) and shiitake substrates (Trichoderma guizhouense T59, 2018, Serbia). Air-dried plant material was subjected to hydro-distillation in a Clevenger type apparatus. The obtained essential oils were dried over anhydrous sodium sulphate. Antifungal activity of the oils was tested using in vitro methods: contact microdilution by using a pathogen spore suspension, and fumigant macrodilution by applying either pathogen mycelial inoculum or spore suspension. Both oils were lethal for all tested species when the contact microdilution method was used at 12.5 μl mL-1, while no fungicidal effect of either oil was found on any tested strain using the fumigant method. In the contact microdilution bio-assay, both oils inhibited T. guizhouense T59 at 3.12 μl mL-1; peppermint oil more strongly suppresed T. pleuroti KM11 (3.12 μl mL-1) than T. pleuroticola KM12 (6.25 μl mL-1), while spearment oil had an opposite effect. Using fumigation, both oils inhibitied mycelial growth of the tested species at 0.16 μl mL-1, with an exception of spearmint oil, which inhibited T. guizhouense T59 at 0.32 μl mL-1 of air phase. Inhibition of spore germination of T. pleuroti KM11 by fumigation was detected at 0.08 (pepermint oil) or 0.016 μl mL-1 (spearmint oil), T. pleuroticola KM12 at 0.016 μl mL-1 (both oils), and T. ghuizouense T59 at 0.32 μl mL-1 (peppermint oil) or inhibition was missing (spearmint oil). The most resistant taxon to both oils was T. guizhoense from shiitake. Both oils showed similar contact effect, while peppermint oil exhibited a slightly stronger fumigant activity than spearmint oil on the tested strains. Peppermint and spearmint oils could be recommended for further in vivo investigation. PB - International Society for Mushroom Science C3 - 20th ISMS CONGRESS Program and Abstract Book T1 - Antifungal activity of peppermint and spearmint essential oils against Trichoderma spp. green mold disease agents of oyster mushroom and shiitake EP - 50 SP - 50 ER -
@conference{ author = "Potočnik, Ivana and Luković, Jelena and Stepanović, Miloš and Todorović, Biljana and Đorđević, Tijana and Đurović-Pejčev, Rada and Milijašević-Marčić, Svetlana", year = "2024", abstract = "Production of oyster mushroom (Pleurotus spp.) and shiitake (Lentinula edodes) is seriously affected by various Trichoderma species causing green mold disease. The purpose of the study was to determine the antifungal activity of essential oils peppermint (Mentha piperita) and spearmint (Mentha spicata), both obtained from plants from Serbia, against Trichoderma strains isolated from Pleurotus ostreatus oyster mushroom (Trichoderma pleuroti KM11 and Trichoderma pleuroticola KM12, 2018, North Macedonia) and shiitake substrates (Trichoderma guizhouense T59, 2018, Serbia). Air-dried plant material was subjected to hydro-distillation in a Clevenger type apparatus. The obtained essential oils were dried over anhydrous sodium sulphate. Antifungal activity of the oils was tested using in vitro methods: contact microdilution by using a pathogen spore suspension, and fumigant macrodilution by applying either pathogen mycelial inoculum or spore suspension. Both oils were lethal for all tested species when the contact microdilution method was used at 12.5 μl mL-1, while no fungicidal effect of either oil was found on any tested strain using the fumigant method. In the contact microdilution bio-assay, both oils inhibited T. guizhouense T59 at 3.12 μl mL-1; peppermint oil more strongly suppresed T. pleuroti KM11 (3.12 μl mL-1) than T. pleuroticola KM12 (6.25 μl mL-1), while spearment oil had an opposite effect. Using fumigation, both oils inhibitied mycelial growth of the tested species at 0.16 μl mL-1, with an exception of spearmint oil, which inhibited T. guizhouense T59 at 0.32 μl mL-1 of air phase. Inhibition of spore germination of T. pleuroti KM11 by fumigation was detected at 0.08 (pepermint oil) or 0.016 μl mL-1 (spearmint oil), T. pleuroticola KM12 at 0.016 μl mL-1 (both oils), and T. ghuizouense T59 at 0.32 μl mL-1 (peppermint oil) or inhibition was missing (spearmint oil). The most resistant taxon to both oils was T. guizhoense from shiitake. Both oils showed similar contact effect, while peppermint oil exhibited a slightly stronger fumigant activity than spearmint oil on the tested strains. Peppermint and spearmint oils could be recommended for further in vivo investigation.", publisher = "International Society for Mushroom Science", journal = "20th ISMS CONGRESS Program and Abstract Book", title = "Antifungal activity of peppermint and spearmint essential oils against Trichoderma spp. green mold disease agents of oyster mushroom and shiitake", pages = "50-50" }
Potočnik, I., Luković, J., Stepanović, M., Todorović, B., Đorđević, T., Đurović-Pejčev, R.,& Milijašević-Marčić, S.. (2024). Antifungal activity of peppermint and spearmint essential oils against Trichoderma spp. green mold disease agents of oyster mushroom and shiitake. in 20th ISMS CONGRESS Program and Abstract Book International Society for Mushroom Science., 50-50.
Potočnik I, Luković J, Stepanović M, Todorović B, Đorđević T, Đurović-Pejčev R, Milijašević-Marčić S. Antifungal activity of peppermint and spearmint essential oils against Trichoderma spp. green mold disease agents of oyster mushroom and shiitake. in 20th ISMS CONGRESS Program and Abstract Book. 2024;:50-50..
Potočnik, Ivana, Luković, Jelena, Stepanović, Miloš, Todorović, Biljana, Đorđević, Tijana, Đurović-Pejčev, Rada, Milijašević-Marčić, Svetlana, "Antifungal activity of peppermint and spearmint essential oils against Trichoderma spp. green mold disease agents of oyster mushroom and shiitake" in 20th ISMS CONGRESS Program and Abstract Book (2024):50-50.