Beneficial microorganisms joining forces to fight green mold in mushrooms
2024
Аутори
Milijašević-Marčić, SvetlanaLuković, Jelena
Šantrić, Ljiljana
Andjelkovic, Nikola
Grujić, Nikola
Drobnjaković, Tanja
Marčić, Dejan
Potočnik, Ivana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The mushroom industry in Serbia is growing rapidly, producing fresh mushrooms of different quality for consumption,
processing or export. A lack of effective chemicals for disease control, and pathogen resistance to pesticides,
consequentially lead to unsuccessful control of the green mold agent, Trichoderma aggressivum. The principles
of sustainable use of pesticides require a change in the attitude of composters and mushroom growers towards
biological disease control. Altering the microbial comunities in compost and casing soil to stimulate the growth
of healthy button mushrooms will hopefully increase mushroom yield and green mold control, and provide a
practice-based understanding of the microbial community-pathogen relationships. The objective of the study was
to implement two indigenous beneficial microorganisms in the cultivation of button mushroom Agaricus bisporus:
Bacillus amyloliquefaciens, strain B-241, a bacterium with fungicidal activity against T. aggressivum, an...d Streptomyces
flavovirens, strain A06, an actinobacterium which stimulates mushroom yield. Interrelationship between
the two beneficial microorganisms was investigated in an experimental mushroom growing room after inoculation
with compost green mold, T. aggressivum f. europaeum, which was added one day after the spawned compost was
placed in boxes (106 conidia/mL per m2). The concentration of beneficial bacterial and actinobacterial suspension
was adjusted to 108 CFU/mL per m2 and applied at seven-day intervals. The synergy factor (SF) was calculated as
a ratio between the observed and expected impact on yield or effectiveness in suppression of disease symptoms.
The results indicated strong synergism in promoting A. bisporus productivity in both uninoculated (SF=1.56) and
inoculated plots (SF=1.62) after combined application of the two beneficial microorganisms. Regarding efficacy
in preventing green mold symptoms, the beneficial bacterium and actinobacterium displayed an additive effect
(SF=1.05). Further investigatation is expected to reveal adequate simultaneous application procedure of the beneficial
organisms in order to maximize biopesticidal/stimulatory effects, and reduce environmental pollution
Кључне речи:
beneficial microorganisms / green mold / mushroomsИзвор:
20th ISMS CONGRESS Program and Abstract Book, 2024, 49-49Издавач:
- International Society for Mushroom Science
Пројекти:
- Micro-Mush - Microbial recipe for edible mushroom production (RS-ScienceFundRS-Zeleni-4848)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200214 (Институт за пестициде и заштиту животне средине, Београд) (RS-MESTD-inst-2020-200214)
Напомена:
- 26-27 February 2024, Las Vegas, USA
Институција
Institut za pesticide i zaštitu životne sredineTY - CONF AU - Milijašević-Marčić, Svetlana AU - Luković, Jelena AU - Šantrić, Ljiljana AU - Andjelkovic, Nikola AU - Grujić, Nikola AU - Drobnjaković, Tanja AU - Marčić, Dejan AU - Potočnik, Ivana PY - 2024 UR - https://ripest.pesting.org.rs/handle/123456789/761 AB - The mushroom industry in Serbia is growing rapidly, producing fresh mushrooms of different quality for consumption, processing or export. A lack of effective chemicals for disease control, and pathogen resistance to pesticides, consequentially lead to unsuccessful control of the green mold agent, Trichoderma aggressivum. The principles of sustainable use of pesticides require a change in the attitude of composters and mushroom growers towards biological disease control. Altering the microbial comunities in compost and casing soil to stimulate the growth of healthy button mushrooms will hopefully increase mushroom yield and green mold control, and provide a practice-based understanding of the microbial community-pathogen relationships. The objective of the study was to implement two indigenous beneficial microorganisms in the cultivation of button mushroom Agaricus bisporus: Bacillus amyloliquefaciens, strain B-241, a bacterium with fungicidal activity against T. aggressivum, and Streptomyces flavovirens, strain A06, an actinobacterium which stimulates mushroom yield. Interrelationship between the two beneficial microorganisms was investigated in an experimental mushroom growing room after inoculation with compost green mold, T. aggressivum f. europaeum, which was added one day after the spawned compost was placed in boxes (106 conidia/mL per m2). The concentration of beneficial bacterial and actinobacterial suspension was adjusted to 108 CFU/mL per m2 and applied at seven-day intervals. The synergy factor (SF) was calculated as a ratio between the observed and expected impact on yield or effectiveness in suppression of disease symptoms. The results indicated strong synergism in promoting A. bisporus productivity in both uninoculated (SF=1.56) and inoculated plots (SF=1.62) after combined application of the two beneficial microorganisms. Regarding efficacy in preventing green mold symptoms, the beneficial bacterium and actinobacterium displayed an additive effect (SF=1.05). Further investigatation is expected to reveal adequate simultaneous application procedure of the beneficial organisms in order to maximize biopesticidal/stimulatory effects, and reduce environmental pollution PB - International Society for Mushroom Science C3 - 20th ISMS CONGRESS Program and Abstract Book T1 - Beneficial microorganisms joining forces to fight green mold in mushrooms EP - 49 SP - 49 ER -
@conference{ author = "Milijašević-Marčić, Svetlana and Luković, Jelena and Šantrić, Ljiljana and Andjelkovic, Nikola and Grujić, Nikola and Drobnjaković, Tanja and Marčić, Dejan and Potočnik, Ivana", year = "2024", abstract = "The mushroom industry in Serbia is growing rapidly, producing fresh mushrooms of different quality for consumption, processing or export. A lack of effective chemicals for disease control, and pathogen resistance to pesticides, consequentially lead to unsuccessful control of the green mold agent, Trichoderma aggressivum. The principles of sustainable use of pesticides require a change in the attitude of composters and mushroom growers towards biological disease control. Altering the microbial comunities in compost and casing soil to stimulate the growth of healthy button mushrooms will hopefully increase mushroom yield and green mold control, and provide a practice-based understanding of the microbial community-pathogen relationships. The objective of the study was to implement two indigenous beneficial microorganisms in the cultivation of button mushroom Agaricus bisporus: Bacillus amyloliquefaciens, strain B-241, a bacterium with fungicidal activity against T. aggressivum, and Streptomyces flavovirens, strain A06, an actinobacterium which stimulates mushroom yield. Interrelationship between the two beneficial microorganisms was investigated in an experimental mushroom growing room after inoculation with compost green mold, T. aggressivum f. europaeum, which was added one day after the spawned compost was placed in boxes (106 conidia/mL per m2). The concentration of beneficial bacterial and actinobacterial suspension was adjusted to 108 CFU/mL per m2 and applied at seven-day intervals. The synergy factor (SF) was calculated as a ratio between the observed and expected impact on yield or effectiveness in suppression of disease symptoms. The results indicated strong synergism in promoting A. bisporus productivity in both uninoculated (SF=1.56) and inoculated plots (SF=1.62) after combined application of the two beneficial microorganisms. Regarding efficacy in preventing green mold symptoms, the beneficial bacterium and actinobacterium displayed an additive effect (SF=1.05). Further investigatation is expected to reveal adequate simultaneous application procedure of the beneficial organisms in order to maximize biopesticidal/stimulatory effects, and reduce environmental pollution", publisher = "International Society for Mushroom Science", journal = "20th ISMS CONGRESS Program and Abstract Book", title = "Beneficial microorganisms joining forces to fight green mold in mushrooms", pages = "49-49" }
Milijašević-Marčić, S., Luković, J., Šantrić, L., Andjelkovic, N., Grujić, N., Drobnjaković, T., Marčić, D.,& Potočnik, I.. (2024). Beneficial microorganisms joining forces to fight green mold in mushrooms. in 20th ISMS CONGRESS Program and Abstract Book International Society for Mushroom Science., 49-49.
Milijašević-Marčić S, Luković J, Šantrić L, Andjelkovic N, Grujić N, Drobnjaković T, Marčić D, Potočnik I. Beneficial microorganisms joining forces to fight green mold in mushrooms. in 20th ISMS CONGRESS Program and Abstract Book. 2024;:49-49..
Milijašević-Marčić, Svetlana, Luković, Jelena, Šantrić, Ljiljana, Andjelkovic, Nikola, Grujić, Nikola, Drobnjaković, Tanja, Marčić, Dejan, Potočnik, Ivana, "Beneficial microorganisms joining forces to fight green mold in mushrooms" in 20th ISMS CONGRESS Program and Abstract Book (2024):49-49.