The use of groEL gene for characterisation of aster yellows phytoplasmas in field collected samples
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Mitrović, JelenaContaldo, Nicoletta
Paltrinieri, Samanta
Mejia, Juan F.
Mori, Nicola
Bertaccini, Assunta
Duduk, Bojan
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Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of 'Candidants Phytoplasma asteris' in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation among strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification.
Keywords:
phytoplasmas / PCR/RFLP analyses / molecular characterization / groEL gene / epidemiologySource:
Bulletin of Insectology, 2011, 64, SUPPL. 1, S17-S18Projects:
- Studies on plant pathogens, arthropods, weeds, and pesticides with a view to developing the methods of biorational plant protection and safe food production (RS-MESTD-Technological Development (TD or TR)-31043)
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Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Mitrović, Jelena AU - Contaldo, Nicoletta AU - Paltrinieri, Samanta AU - Mejia, Juan F. AU - Mori, Nicola AU - Bertaccini, Assunta AU - Duduk, Bojan PY - 2011 UR - https://ripest.pesting.org.rs/handle/123456789/200 AB - Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of 'Candidants Phytoplasma asteris' in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation among strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification. T2 - Bulletin of Insectology T1 - The use of groEL gene for characterisation of aster yellows phytoplasmas in field collected samples EP - S18 IS - SUPPL. 1 SP - S17 VL - 64 UR - conv_681 ER -
@article{ author = "Mitrović, Jelena and Contaldo, Nicoletta and Paltrinieri, Samanta and Mejia, Juan F. and Mori, Nicola and Bertaccini, Assunta and Duduk, Bojan", year = "2011", abstract = "Amplification of fragments containing phytoplasma groEL gene sequence with a newly designed nested PCR system allowed to specifically detect the presence of 'Candidants Phytoplasma asteris' in reference strains as well as in samples field collected and maintained as dry/freeze dried nucleic acids. After RFLP analyses it was possible to confirm further finer differentiation among strains enclosed in subgroup 16SrI-B by previous ribosomal gene classification.", journal = "Bulletin of Insectology", title = "The use of groEL gene for characterisation of aster yellows phytoplasmas in field collected samples", pages = "S18-S17", number = "SUPPL. 1", volume = "64", url = "conv_681" }
Mitrović, J., Contaldo, N., Paltrinieri, S., Mejia, J. F., Mori, N., Bertaccini, A.,& Duduk, B.. (2011). The use of groEL gene for characterisation of aster yellows phytoplasmas in field collected samples. in Bulletin of Insectology, 64(SUPPL. 1), S17-S18. conv_681
Mitrović J, Contaldo N, Paltrinieri S, Mejia JF, Mori N, Bertaccini A, Duduk B. The use of groEL gene for characterisation of aster yellows phytoplasmas in field collected samples. in Bulletin of Insectology. 2011;64(SUPPL. 1):S17-S18. conv_681 .
Mitrović, Jelena, Contaldo, Nicoletta, Paltrinieri, Samanta, Mejia, Juan F., Mori, Nicola, Bertaccini, Assunta, Duduk, Bojan, "The use of groEL gene for characterisation of aster yellows phytoplasmas in field collected samples" in Bulletin of Insectology, 64, no. SUPPL. 1 (2011):S17-S18, conv_681 .