Genetic layout and genetic structure of the greenhouse whitefly Trialeurodes vaporariorum from Serbia
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2019
Authors
Prijović, Mirjana![](/themes/Mirageripest/images/orcid.png)
Aleksić, Jelena
Banjanac, Tijana
Drobnjaković, Tanja
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Marčić, Dejan
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Contributors
Vasiljević, BrankaPatenković, Aleksandra
Nikolić, Nađa
Conference object (Published version)
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Show full item recordAbstract
Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), a haplo-diploid sap
feeding insect species, is a vector of many plant viruses and a serious polyphagous pest of
greenhouse-grown vegetables and ornamental plants worldwide. To explore the populations
of this pest and recognize potential invasion routes, the genetic diversity and genetic structure
of T. vaporariorum from Serbia were analysed using six microsatellite loci. PCR multiplex
conditions were optimized and tested using 88 T. vaporariorum females collected from
natural populations in 10 sampling locations in Serbia: Apatin (AP), Beograd (BG), Kovin (KO),
Negotin (NE), Pirot (PI), Smederevska Palanka (SP), Subotica (SU), Svilajnac (SV), Trbušan (TR)
and Vranjska Banja (VB). Observed heterozygosity ranged from 0.105 to 0.796, deviations of
the frequency of genotypes from the expected Hardy–Weinberg equilibrium were detected in
all populations. The values of the Fst parameters ranged from 0.011 between populatio...ns of
AP and SU, up to 0.286 among the SV and BG populations. The obtained results for Fst values
were used to explore genetic structure using several analyzes: PAST, Principal Coordinate
Analysis – PCoA and SAMOVA. The analyzed T. vaporariorum populations were grouped in
three distinct genetic clusters based on these population-genetic analyses: (1) BG, (2) AP,
KO, SP, TR, VB and SU, and (3) SV, NE and PI. Also, BOTTLENECK identified reductions in
effective size of SP population in the recent past and the significant heterozygosity deficiency
was detected in the four populations VB, SU, BG and NE. These results demonstrate that
populations of T. vaporariorum in Serbia show significant genetic differentiation, indicating
the likelihood of multiple introductions of this important pest into Serbia, as well as the
influence of crop protection and pest management on their genetic variability.
Keywords:
greenhouse whitefly / genetic differentiation / genetic structureSource:
6th Congress of the Serbian Genetic Society - Book of Abstracts, 2019, 176-176Publisher:
- Belgrade : Serbian Genetic Society
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Institution
Institut za pesticide i zaštitu životne sredineTY - CONF AU - Prijović, Mirjana AU - Aleksić, Jelena AU - Banjanac, Tijana AU - Drobnjaković, Tanja AU - Marčić, Dejan PY - 2019 UR - https://ripest.pesting.org.rs/handle/123456789/627 AB - Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), a haplo-diploid sap feeding insect species, is a vector of many plant viruses and a serious polyphagous pest of greenhouse-grown vegetables and ornamental plants worldwide. To explore the populations of this pest and recognize potential invasion routes, the genetic diversity and genetic structure of T. vaporariorum from Serbia were analysed using six microsatellite loci. PCR multiplex conditions were optimized and tested using 88 T. vaporariorum females collected from natural populations in 10 sampling locations in Serbia: Apatin (AP), Beograd (BG), Kovin (KO), Negotin (NE), Pirot (PI), Smederevska Palanka (SP), Subotica (SU), Svilajnac (SV), Trbušan (TR) and Vranjska Banja (VB). Observed heterozygosity ranged from 0.105 to 0.796, deviations of the frequency of genotypes from the expected Hardy–Weinberg equilibrium were detected in all populations. The values of the Fst parameters ranged from 0.011 between populations of AP and SU, up to 0.286 among the SV and BG populations. The obtained results for Fst values were used to explore genetic structure using several analyzes: PAST, Principal Coordinate Analysis – PCoA and SAMOVA. The analyzed T. vaporariorum populations were grouped in three distinct genetic clusters based on these population-genetic analyses: (1) BG, (2) AP, KO, SP, TR, VB and SU, and (3) SV, NE and PI. Also, BOTTLENECK identified reductions in effective size of SP population in the recent past and the significant heterozygosity deficiency was detected in the four populations VB, SU, BG and NE. These results demonstrate that populations of T. vaporariorum in Serbia show significant genetic differentiation, indicating the likelihood of multiple introductions of this important pest into Serbia, as well as the influence of crop protection and pest management on their genetic variability. PB - Belgrade : Serbian Genetic Society C3 - 6th Congress of the Serbian Genetic Society - Book of Abstracts T1 - Genetic layout and genetic structure of the greenhouse whitefly Trialeurodes vaporariorum from Serbia EP - 176 SP - 176 ER -
@conference{ author = "Prijović, Mirjana and Aleksić, Jelena and Banjanac, Tijana and Drobnjaković, Tanja and Marčić, Dejan", year = "2019", abstract = "Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae), a haplo-diploid sap feeding insect species, is a vector of many plant viruses and a serious polyphagous pest of greenhouse-grown vegetables and ornamental plants worldwide. To explore the populations of this pest and recognize potential invasion routes, the genetic diversity and genetic structure of T. vaporariorum from Serbia were analysed using six microsatellite loci. PCR multiplex conditions were optimized and tested using 88 T. vaporariorum females collected from natural populations in 10 sampling locations in Serbia: Apatin (AP), Beograd (BG), Kovin (KO), Negotin (NE), Pirot (PI), Smederevska Palanka (SP), Subotica (SU), Svilajnac (SV), Trbušan (TR) and Vranjska Banja (VB). Observed heterozygosity ranged from 0.105 to 0.796, deviations of the frequency of genotypes from the expected Hardy–Weinberg equilibrium were detected in all populations. The values of the Fst parameters ranged from 0.011 between populations of AP and SU, up to 0.286 among the SV and BG populations. The obtained results for Fst values were used to explore genetic structure using several analyzes: PAST, Principal Coordinate Analysis – PCoA and SAMOVA. The analyzed T. vaporariorum populations were grouped in three distinct genetic clusters based on these population-genetic analyses: (1) BG, (2) AP, KO, SP, TR, VB and SU, and (3) SV, NE and PI. Also, BOTTLENECK identified reductions in effective size of SP population in the recent past and the significant heterozygosity deficiency was detected in the four populations VB, SU, BG and NE. These results demonstrate that populations of T. vaporariorum in Serbia show significant genetic differentiation, indicating the likelihood of multiple introductions of this important pest into Serbia, as well as the influence of crop protection and pest management on their genetic variability.", publisher = "Belgrade : Serbian Genetic Society", journal = "6th Congress of the Serbian Genetic Society - Book of Abstracts", title = "Genetic layout and genetic structure of the greenhouse whitefly Trialeurodes vaporariorum from Serbia", pages = "176-176" }
Prijović, M., Aleksić, J., Banjanac, T., Drobnjaković, T.,& Marčić, D.. (2019). Genetic layout and genetic structure of the greenhouse whitefly Trialeurodes vaporariorum from Serbia. in 6th Congress of the Serbian Genetic Society - Book of Abstracts Belgrade : Serbian Genetic Society., 176-176.
Prijović M, Aleksić J, Banjanac T, Drobnjaković T, Marčić D. Genetic layout and genetic structure of the greenhouse whitefly Trialeurodes vaporariorum from Serbia. in 6th Congress of the Serbian Genetic Society - Book of Abstracts. 2019;:176-176..
Prijović, Mirjana, Aleksić, Jelena, Banjanac, Tijana, Drobnjaković, Tanja, Marčić, Dejan, "Genetic layout and genetic structure of the greenhouse whitefly Trialeurodes vaporariorum from Serbia" in 6th Congress of the Serbian Genetic Society - Book of Abstracts (2019):176-176.