Efficacy of difenacoum against house mice carrying tyr139cys and leu128ser/tyr139cys vkor-variants
2015
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The VKOR-variants Leu128Ser and Tyr139Cys are the chief cause of
anticoagulant resistance occurring in house mice. Recent studies have revealed
that the two polymorphisms occur simultaneously in the vkorc1 gene exon 3.
Difenacoum efficacy against animals with that type of VKOR-variant had not
been determined in house mice before the present study.
Fifteen house mice were used in a no-choice feeding test, according to
standard EPPO (2004) methodology. The animals were fed for 21 days on
baits that contained difenacoum 0.005%. Seven months earlier, all animals
had survived a 21-day long bromadiolone (0.005%) no-choice feeding test, and
sequencing of their vkorc1 gene had revealed the presence of Tyr139Cys in
eight and Leu1258Ser/Tyr139Cys VKOR-variant in seven animals. In our present
study, all animals were given daily fresh baits and their daily consumption was
measured. Water was available ad lib.
None of the animals survived the no-choice feeding test. All animals carryin...g
Leu128Ser/Tyr139Cys died after eight days, and all carrying Tyr139Cys died
after twelve days. Animals with the combination Leu128Ser/Tyr139Cys had a
greater tolerance to difenacoum than Tyr139Cys-carrying animals as the former
had 0.74 gg-1 bw average sum bait consumption and the latter 0.63 gg-1 bw.
Our preliminary tests showed that difenacoum 0.005% was effective against
house mice carrying Tyr139Cys and Leu128Ser/Tyr139Cys. Tests involving
more animals are planned to clarify the level of tolerance of house mice to
difenacoum and the effect of the former treatment with bromadiolone.
Ključne reči:
difenacoum / house mouse / TYR139CYS / LEU128SER/TYR139CYS / VKOR variantsIzvor:
10th European Vertebrate Pest Management Conference - Abstracts, 2015, 123-123Napomena:
- Seville, 21 - 25 September 2015
Institucija
Institut za pesticide i zaštitu životne sredineTY - CONF AU - Šćepović, Tanja AU - Jokić, Goran AU - Esther, Alexandra AU - Đedović, Suzana AU - Vukša, Petar AU - Vukša, Marina PY - 2015 UR - https://ripest.pesting.org.rs/handle/123456789/718 AB - The VKOR-variants Leu128Ser and Tyr139Cys are the chief cause of anticoagulant resistance occurring in house mice. Recent studies have revealed that the two polymorphisms occur simultaneously in the vkorc1 gene exon 3. Difenacoum efficacy against animals with that type of VKOR-variant had not been determined in house mice before the present study. Fifteen house mice were used in a no-choice feeding test, according to standard EPPO (2004) methodology. The animals were fed for 21 days on baits that contained difenacoum 0.005%. Seven months earlier, all animals had survived a 21-day long bromadiolone (0.005%) no-choice feeding test, and sequencing of their vkorc1 gene had revealed the presence of Tyr139Cys in eight and Leu1258Ser/Tyr139Cys VKOR-variant in seven animals. In our present study, all animals were given daily fresh baits and their daily consumption was measured. Water was available ad lib. None of the animals survived the no-choice feeding test. All animals carrying Leu128Ser/Tyr139Cys died after eight days, and all carrying Tyr139Cys died after twelve days. Animals with the combination Leu128Ser/Tyr139Cys had a greater tolerance to difenacoum than Tyr139Cys-carrying animals as the former had 0.74 gg-1 bw average sum bait consumption and the latter 0.63 gg-1 bw. Our preliminary tests showed that difenacoum 0.005% was effective against house mice carrying Tyr139Cys and Leu128Ser/Tyr139Cys. Tests involving more animals are planned to clarify the level of tolerance of house mice to difenacoum and the effect of the former treatment with bromadiolone. C3 - 10th European Vertebrate Pest Management Conference - Abstracts T1 - Efficacy of difenacoum against house mice carrying tyr139cys and leu128ser/tyr139cys vkor-variants EP - 123 SP - 123 ER -
@conference{ author = "Šćepović, Tanja and Jokić, Goran and Esther, Alexandra and Đedović, Suzana and Vukša, Petar and Vukša, Marina", year = "2015", abstract = "The VKOR-variants Leu128Ser and Tyr139Cys are the chief cause of anticoagulant resistance occurring in house mice. Recent studies have revealed that the two polymorphisms occur simultaneously in the vkorc1 gene exon 3. Difenacoum efficacy against animals with that type of VKOR-variant had not been determined in house mice before the present study. Fifteen house mice were used in a no-choice feeding test, according to standard EPPO (2004) methodology. The animals were fed for 21 days on baits that contained difenacoum 0.005%. Seven months earlier, all animals had survived a 21-day long bromadiolone (0.005%) no-choice feeding test, and sequencing of their vkorc1 gene had revealed the presence of Tyr139Cys in eight and Leu1258Ser/Tyr139Cys VKOR-variant in seven animals. In our present study, all animals were given daily fresh baits and their daily consumption was measured. Water was available ad lib. None of the animals survived the no-choice feeding test. All animals carrying Leu128Ser/Tyr139Cys died after eight days, and all carrying Tyr139Cys died after twelve days. Animals with the combination Leu128Ser/Tyr139Cys had a greater tolerance to difenacoum than Tyr139Cys-carrying animals as the former had 0.74 gg-1 bw average sum bait consumption and the latter 0.63 gg-1 bw. Our preliminary tests showed that difenacoum 0.005% was effective against house mice carrying Tyr139Cys and Leu128Ser/Tyr139Cys. Tests involving more animals are planned to clarify the level of tolerance of house mice to difenacoum and the effect of the former treatment with bromadiolone.", journal = "10th European Vertebrate Pest Management Conference - Abstracts", title = "Efficacy of difenacoum against house mice carrying tyr139cys and leu128ser/tyr139cys vkor-variants", pages = "123-123" }
Šćepović, T., Jokić, G., Esther, A., Đedović, S., Vukša, P.,& Vukša, M.. (2015). Efficacy of difenacoum against house mice carrying tyr139cys and leu128ser/tyr139cys vkor-variants. in 10th European Vertebrate Pest Management Conference - Abstracts, 123-123.
Šćepović T, Jokić G, Esther A, Đedović S, Vukša P, Vukša M. Efficacy of difenacoum against house mice carrying tyr139cys and leu128ser/tyr139cys vkor-variants. in 10th European Vertebrate Pest Management Conference - Abstracts. 2015;:123-123..
Šćepović, Tanja, Jokić, Goran, Esther, Alexandra, Đedović, Suzana, Vukša, Petar, Vukša, Marina, "Efficacy of difenacoum against house mice carrying tyr139cys and leu128ser/tyr139cys vkor-variants" in 10th European Vertebrate Pest Management Conference - Abstracts (2015):123-123.