Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava
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2009
Аутори
Alvarez, ElizabethMejia, Juan F.
Llano, German A.
Loke, John B.
Calari, Alberto
Duduk, Bojan
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Bertaccini, Assunta
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Чланак у часопису (Објављена верзија)
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Метаподаци
Приказ свих података о документуАпстракт
Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction (PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas were identified as group 16SrIII strains by restriction fragment length polymorphism (RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers. Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described previously in group 16SrIII and, on this basis, the strains were tentatively ass...igned to new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia.
Извор:
Plant Disease, 2009, 93, 11, 1139-1145Издавач:
- AMER PHYTOPATHOLOGICAL SOC, ST PAUL
DOI: 10.1094/PDIS-93-11-1139
ISSN: 0191-2917
PubMed: 30754574
WoS: 000271045000007
Scopus: 2-s2.0-70449635975
Институција
Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Alvarez, Elizabeth AU - Mejia, Juan F. AU - Llano, German A. AU - Loke, John B. AU - Calari, Alberto AU - Duduk, Bojan AU - Bertaccini, Assunta PY - 2009 UR - https://ripest.pesting.org.rs/handle/123456789/132 AB - Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction (PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas were identified as group 16SrIII strains by restriction fragment length polymorphism (RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers. Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described previously in group 16SrIII and, on this basis, the strains were tentatively assigned to new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia. PB - AMER PHYTOPATHOLOGICAL SOC, ST PAUL T2 - Plant Disease T1 - Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava EP - 1145 IS - 11 SP - 1139 VL - 93 DO - 10.1094/PDIS-93-11-1139 UR - conv_358 ER -
@article{ author = "Alvarez, Elizabeth and Mejia, Juan F. and Llano, German A. and Loke, John B. and Calari, Alberto and Duduk, Bojan and Bertaccini, Assunta", year = "2009", abstract = "Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction (PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas were identified as group 16SrIII strains by restriction fragment length polymorphism (RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers. Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described previously in group 16SrIII and, on this basis, the strains were tentatively assigned to new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia.", publisher = "AMER PHYTOPATHOLOGICAL SOC, ST PAUL", journal = "Plant Disease", title = "Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava", pages = "1145-1139", number = "11", volume = "93", doi = "10.1094/PDIS-93-11-1139", url = "conv_358" }
Alvarez, E., Mejia, J. F., Llano, G. A., Loke, J. B., Calari, A., Duduk, B.,& Bertaccini, A.. (2009). Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava. in Plant Disease AMER PHYTOPATHOLOGICAL SOC, ST PAUL., 93(11), 1139-1145. https://doi.org/10.1094/PDIS-93-11-1139 conv_358
Alvarez E, Mejia JF, Llano GA, Loke JB, Calari A, Duduk B, Bertaccini A. Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava. in Plant Disease. 2009;93(11):1139-1145. doi:10.1094/PDIS-93-11-1139 conv_358 .
Alvarez, Elizabeth, Mejia, Juan F., Llano, German A., Loke, John B., Calari, Alberto, Duduk, Bojan, Bertaccini, Assunta, "Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava" in Plant Disease, 93, no. 11 (2009):1139-1145, https://doi.org/10.1094/PDIS-93-11-1139 ., conv_358 .