Antifungal effect of Bacillus subtilis B6 strain on Monilinia fructicola
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2019
Аутори
Hrustić, Jovana![](/themes/Mirageripest/images/orcid.png)
Mihajlović, Milica
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Grahovac, Mila
Stevanović, Marija
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Delibašić, Goran
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Gašić, Slavica
Tanović, Brankica
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Конференцијски прилог (Објављена верзија)
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Метаподаци
Приказ свих података о документуАпстракт
Brown rot caused by Monilinia spp. is one of the major factors limiting the storage period and market life of stone and pome fruits. Control of postharvest decay is currently based on the use of synthetic fungicides during bloom and preharvest periods, while postharvest use of fungicides is restricted in EU countries, as well as in Serbia. In recent years, the use of environmentally friendly approaches, such as biological control agents, has increased continuously due to public concerns over the risks of pesticide residues remaining in food and their negative impact on the environment. One of the most significant practical constraints in the use of biocontrol products is the required development of their shelf-stable formulations.
The aim of this study was to test antifungal properties of numerous bacterial strains from Serbia against Monilinia fructicola, to develop a suspo-emulsion (SE) formulation, and to test its efficacy in apple protection against one of the most damaging brown ...fruit rot pathogens. We conducted in vitro screening of many bacterial isolates originating from different substrates for their antifungal properties against a M. fructicola isolate as a model organism. In vitro antagonistic activity assays were performed in 90 mm Petri plates using the wells technique. Based on antagonistic effects in vitro, seven of 108 isolates were chosen for further studies. The isolates were identified to the species level based on morphological and molecular characteristics. One the most promising antagonistic strains was then selected to develop a SE biopesticide formulation and test its bioactivity in vivo on apple fruits cv. Golden Delicious. The results showed that the investigated bacteria exhibited antifungal properties against M. fructicola in vitro, and that the B6 strain of Bacillus subtilis qualifies to be selected as the best for further testing in vivo. The experiment on wound-inoculated apple fruits showed that the SE formulation of B6 strain was as effective as a reference synthetic fungicide and that its effectiveness remained stable for at least 24 hours. On inoculated apple fruits, the 10% concentration of SE formulation was the most effective, achieving 80% efficacy compared to the control. This work confirmed a great potential of the isolated bacterial strain and its formulation for biological control of brown rot in apple fruit.
Кључне речи:
biological control / biopesticides / brown rot / antimicrobial activity / apple fruitИзвор:
Vth International Symposium on Postharvest Pathology - Book of abstracts, 2019Издавач:
- Liege : Liege Universite
- St. Paul : ISPP
- Leuven : ISHS
Пројекти:
- Развој интегрисаних система управљања штетним организмима у биљној производњи са циљем превазилажења резистентности и унапређења квалитета и безбедности хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
Напомена:
- From Consumer to Laboratory: Sustainable Approaches to Managing Postharvest Pathogens, 19 – 24 May, 2019, Liège, Belgium
Институција
Institut za pesticide i zaštitu životne sredineTY - CONF AU - Hrustić, Jovana AU - Mihajlović, Milica AU - Grahovac, Mila AU - Stevanović, Marija AU - Delibašić, Goran AU - Gašić, Slavica AU - Tanović, Brankica PY - 2019 UR - https://ripest.pesting.org.rs/handle/123456789/635 AB - Brown rot caused by Monilinia spp. is one of the major factors limiting the storage period and market life of stone and pome fruits. Control of postharvest decay is currently based on the use of synthetic fungicides during bloom and preharvest periods, while postharvest use of fungicides is restricted in EU countries, as well as in Serbia. In recent years, the use of environmentally friendly approaches, such as biological control agents, has increased continuously due to public concerns over the risks of pesticide residues remaining in food and their negative impact on the environment. One of the most significant practical constraints in the use of biocontrol products is the required development of their shelf-stable formulations. The aim of this study was to test antifungal properties of numerous bacterial strains from Serbia against Monilinia fructicola, to develop a suspo-emulsion (SE) formulation, and to test its efficacy in apple protection against one of the most damaging brown fruit rot pathogens. We conducted in vitro screening of many bacterial isolates originating from different substrates for their antifungal properties against a M. fructicola isolate as a model organism. In vitro antagonistic activity assays were performed in 90 mm Petri plates using the wells technique. Based on antagonistic effects in vitro, seven of 108 isolates were chosen for further studies. The isolates were identified to the species level based on morphological and molecular characteristics. One the most promising antagonistic strains was then selected to develop a SE biopesticide formulation and test its bioactivity in vivo on apple fruits cv. Golden Delicious. The results showed that the investigated bacteria exhibited antifungal properties against M. fructicola in vitro, and that the B6 strain of Bacillus subtilis qualifies to be selected as the best for further testing in vivo. The experiment on wound-inoculated apple fruits showed that the SE formulation of B6 strain was as effective as a reference synthetic fungicide and that its effectiveness remained stable for at least 24 hours. On inoculated apple fruits, the 10% concentration of SE formulation was the most effective, achieving 80% efficacy compared to the control. This work confirmed a great potential of the isolated bacterial strain and its formulation for biological control of brown rot in apple fruit. PB - Liege : Liege Universite PB - St. Paul : ISPP PB - Leuven : ISHS C3 - Vth International Symposium on Postharvest Pathology - Book of abstracts T1 - Antifungal effect of Bacillus subtilis B6 strain on Monilinia fructicola ER -
@conference{ author = "Hrustić, Jovana and Mihajlović, Milica and Grahovac, Mila and Stevanović, Marija and Delibašić, Goran and Gašić, Slavica and Tanović, Brankica", year = "2019", abstract = "Brown rot caused by Monilinia spp. is one of the major factors limiting the storage period and market life of stone and pome fruits. Control of postharvest decay is currently based on the use of synthetic fungicides during bloom and preharvest periods, while postharvest use of fungicides is restricted in EU countries, as well as in Serbia. In recent years, the use of environmentally friendly approaches, such as biological control agents, has increased continuously due to public concerns over the risks of pesticide residues remaining in food and their negative impact on the environment. One of the most significant practical constraints in the use of biocontrol products is the required development of their shelf-stable formulations. The aim of this study was to test antifungal properties of numerous bacterial strains from Serbia against Monilinia fructicola, to develop a suspo-emulsion (SE) formulation, and to test its efficacy in apple protection against one of the most damaging brown fruit rot pathogens. We conducted in vitro screening of many bacterial isolates originating from different substrates for their antifungal properties against a M. fructicola isolate as a model organism. In vitro antagonistic activity assays were performed in 90 mm Petri plates using the wells technique. Based on antagonistic effects in vitro, seven of 108 isolates were chosen for further studies. The isolates were identified to the species level based on morphological and molecular characteristics. One the most promising antagonistic strains was then selected to develop a SE biopesticide formulation and test its bioactivity in vivo on apple fruits cv. Golden Delicious. The results showed that the investigated bacteria exhibited antifungal properties against M. fructicola in vitro, and that the B6 strain of Bacillus subtilis qualifies to be selected as the best for further testing in vivo. The experiment on wound-inoculated apple fruits showed that the SE formulation of B6 strain was as effective as a reference synthetic fungicide and that its effectiveness remained stable for at least 24 hours. On inoculated apple fruits, the 10% concentration of SE formulation was the most effective, achieving 80% efficacy compared to the control. This work confirmed a great potential of the isolated bacterial strain and its formulation for biological control of brown rot in apple fruit.", publisher = "Liege : Liege Universite, St. Paul : ISPP, Leuven : ISHS", journal = "Vth International Symposium on Postharvest Pathology - Book of abstracts", title = "Antifungal effect of Bacillus subtilis B6 strain on Monilinia fructicola" }
Hrustić, J., Mihajlović, M., Grahovac, M., Stevanović, M., Delibašić, G., Gašić, S.,& Tanović, B.. (2019). Antifungal effect of Bacillus subtilis B6 strain on Monilinia fructicola. in Vth International Symposium on Postharvest Pathology - Book of abstracts Liege : Liege Universite..
Hrustić J, Mihajlović M, Grahovac M, Stevanović M, Delibašić G, Gašić S, Tanović B. Antifungal effect of Bacillus subtilis B6 strain on Monilinia fructicola. in Vth International Symposium on Postharvest Pathology - Book of abstracts. 2019;..
Hrustić, Jovana, Mihajlović, Milica, Grahovac, Mila, Stevanović, Marija, Delibašić, Goran, Gašić, Slavica, Tanović, Brankica, "Antifungal effect of Bacillus subtilis B6 strain on Monilinia fructicola" in Vth International Symposium on Postharvest Pathology - Book of abstracts (2019).