To meet modern taxonomical approach: developing molecular tools using short mitochondrial fragments from archival specimens
2015
Преузимање 🢃
Аутори
Jović, JelenaMitrović, Milana
Cvrković, Tatjana
Jakovljević, Miljana
Krstić, Oliver
Kosovac, Andrea
Toševski, Ivo
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Biodiversity protection, preservation and inventorization are leading concepts in modern biology. However, species identification and precise recognition of taxa still remain the key problems in basic and applied studies. It is indisputable that during the last 250 years, traditional taxonomy accumulated knowledge that was incorporated into every aspect of current research of biodiversity. Thus, current taxonomy is based exclusively on the typological species concept, following the International Codes of Nomenclature (Zoological, Botanical, Bacteria, etc.). Barcoding initiative lent insight into potentials of molecular methods to provide better taxonomic resolution in species recognition efforts. This is especially true after declining number of traditional taxonomic practitioners in past 30 years, which launched the molecular tools application in species characterization as a certain approach in future studies.
If we consider determination of any organism at the species level to be p...rimarily based on traditional taxonomic methods, applying molecular methods to define genetic content of barcoding region (mtCOI gene) of archived type specimens should be treated as a method of choice for precise identification. Heaving in mind that DNA in archived specimens is usually more or less defragmented at a certain degree, employing methods of PCR amplification and sequencing of short mitochondrial fragments my lead to clarification of nomenclatural doubts accumulated in the past. In addition, the use of prior genetic information to identify short phylogenetically informative amplicons offers a useful approach for molecular phylogenetic analyses especially when de novo collected material is not available or feasible.
Кључне речи:
barcoding region / short mitochondrial fragments / archived type specimens / molecular phylogenyИзвор:
13th International Congress on the Zoogeography and Ecology of Greece and Adjacent Regions - Abstracts, 2015, 47-47Издавач:
- Irakleio : Hellenic Zoological Society
Напомена:
- 7-11 October 2015, Irakleio, Crete
Институција
Institut za pesticide i zaštitu životne sredineTY - CONF AU - Jović, Jelena AU - Mitrović, Milana AU - Cvrković, Tatjana AU - Jakovljević, Miljana AU - Krstić, Oliver AU - Kosovac, Andrea AU - Toševski, Ivo PY - 2015 UR - https://ripest.pesting.org.rs/handle/123456789/733 AB - Biodiversity protection, preservation and inventorization are leading concepts in modern biology. However, species identification and precise recognition of taxa still remain the key problems in basic and applied studies. It is indisputable that during the last 250 years, traditional taxonomy accumulated knowledge that was incorporated into every aspect of current research of biodiversity. Thus, current taxonomy is based exclusively on the typological species concept, following the International Codes of Nomenclature (Zoological, Botanical, Bacteria, etc.). Barcoding initiative lent insight into potentials of molecular methods to provide better taxonomic resolution in species recognition efforts. This is especially true after declining number of traditional taxonomic practitioners in past 30 years, which launched the molecular tools application in species characterization as a certain approach in future studies. If we consider determination of any organism at the species level to be primarily based on traditional taxonomic methods, applying molecular methods to define genetic content of barcoding region (mtCOI gene) of archived type specimens should be treated as a method of choice for precise identification. Heaving in mind that DNA in archived specimens is usually more or less defragmented at a certain degree, employing methods of PCR amplification and sequencing of short mitochondrial fragments my lead to clarification of nomenclatural doubts accumulated in the past. In addition, the use of prior genetic information to identify short phylogenetically informative amplicons offers a useful approach for molecular phylogenetic analyses especially when de novo collected material is not available or feasible. PB - Irakleio : Hellenic Zoological Society C3 - 13th International Congress on the Zoogeography and Ecology of Greece and Adjacent Regions - Abstracts T1 - To meet modern taxonomical approach: developing molecular tools using short mitochondrial fragments from archival specimens EP - 47 SP - 47 ER -
@conference{ author = "Jović, Jelena and Mitrović, Milana and Cvrković, Tatjana and Jakovljević, Miljana and Krstić, Oliver and Kosovac, Andrea and Toševski, Ivo", year = "2015", abstract = "Biodiversity protection, preservation and inventorization are leading concepts in modern biology. However, species identification and precise recognition of taxa still remain the key problems in basic and applied studies. It is indisputable that during the last 250 years, traditional taxonomy accumulated knowledge that was incorporated into every aspect of current research of biodiversity. Thus, current taxonomy is based exclusively on the typological species concept, following the International Codes of Nomenclature (Zoological, Botanical, Bacteria, etc.). Barcoding initiative lent insight into potentials of molecular methods to provide better taxonomic resolution in species recognition efforts. This is especially true after declining number of traditional taxonomic practitioners in past 30 years, which launched the molecular tools application in species characterization as a certain approach in future studies. If we consider determination of any organism at the species level to be primarily based on traditional taxonomic methods, applying molecular methods to define genetic content of barcoding region (mtCOI gene) of archived type specimens should be treated as a method of choice for precise identification. Heaving in mind that DNA in archived specimens is usually more or less defragmented at a certain degree, employing methods of PCR amplification and sequencing of short mitochondrial fragments my lead to clarification of nomenclatural doubts accumulated in the past. In addition, the use of prior genetic information to identify short phylogenetically informative amplicons offers a useful approach for molecular phylogenetic analyses especially when de novo collected material is not available or feasible.", publisher = "Irakleio : Hellenic Zoological Society", journal = "13th International Congress on the Zoogeography and Ecology of Greece and Adjacent Regions - Abstracts", title = "To meet modern taxonomical approach: developing molecular tools using short mitochondrial fragments from archival specimens", pages = "47-47" }
Jović, J., Mitrović, M., Cvrković, T., Jakovljević, M., Krstić, O., Kosovac, A.,& Toševski, I.. (2015). To meet modern taxonomical approach: developing molecular tools using short mitochondrial fragments from archival specimens. in 13th International Congress on the Zoogeography and Ecology of Greece and Adjacent Regions - Abstracts Irakleio : Hellenic Zoological Society., 47-47.
Jović J, Mitrović M, Cvrković T, Jakovljević M, Krstić O, Kosovac A, Toševski I. To meet modern taxonomical approach: developing molecular tools using short mitochondrial fragments from archival specimens. in 13th International Congress on the Zoogeography and Ecology of Greece and Adjacent Regions - Abstracts. 2015;:47-47..
Jović, Jelena, Mitrović, Milana, Cvrković, Tatjana, Jakovljević, Miljana, Krstić, Oliver, Kosovac, Andrea, Toševski, Ivo, "To meet modern taxonomical approach: developing molecular tools using short mitochondrial fragments from archival specimens" in 13th International Congress on the Zoogeography and Ecology of Greece and Adjacent Regions - Abstracts (2015):47-47.