Screening of antagonistic activity of selected microorganisms against apple rot pathogens
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Grahovac, MilaBalaž, Jelica
Grahovac, Jovana
Dodić, Jelena M.
Tanović, Brankica
Hrustić, Jovana
Tadijan, Ivana Z.
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Biological control of plant pathogens is considered an attractive alternative to chemical-based treatments because it has a minimal impact on the environment. In this study, in vitro antifungal efficiency of four microorganisms (Streptomyces hygroscopicus, Saccharomyces cervisae, Bacillus cereus and Leuconostoc mesenteroides) against causal agents of apple fruit rot, was investigated. Isolates of Colletotrichum acutatum, Colletotrichum gloeosporioides and Fusarium avenaceum were obtained from apple fruit samples expressing rot symptoms. Apple samples were collected during 2012 from Ultra Low Oxygen storages in Vojvodina Province, Serbia. The test microorganisms were identified according to pathogenic, morphological and ecological characteristics, and the identification of Colletotrichum isolates was confirmed by polimerase chain reaction (PCR). Antifungal effect was tested using wells method and the obtained data were processed by factorial ANOVA. Duncan's multiple range test was used ...to test significance of differences (p lt = 0.05) between mean values of measured radius of inhibition zones. Activity of S. cervisae, B. cereus and L. mesenteroides was not satisfactory. On the other hand, S. hygroscopicus expressed the strongest antagonism against all tested fungal isolates. The studied isolate of S. hygroscopicus showed significant antagonistic properties against storage pathogens of apple fruits identifying itself as promising biocontrol agent for commercial use.
Keywords:
Streptomyces hygroscopicus / biocontrol / apple storage pathogens / antagonistsSource:
Romanian Biotechnological Letters, 2014, 19, 1, 8959-8965Projects:
- Provincial Secretariat for Science and Technological Development of Autonomous Province of Vojvodina, Republic of Serbia [114-451-3669/2012, 114-17-20/2012]
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Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Grahovac, Mila AU - Balaž, Jelica AU - Grahovac, Jovana AU - Dodić, Jelena M. AU - Tanović, Brankica AU - Hrustić, Jovana AU - Tadijan, Ivana Z. PY - 2014 UR - https://ripest.pesting.org.rs/handle/123456789/317 AB - Biological control of plant pathogens is considered an attractive alternative to chemical-based treatments because it has a minimal impact on the environment. In this study, in vitro antifungal efficiency of four microorganisms (Streptomyces hygroscopicus, Saccharomyces cervisae, Bacillus cereus and Leuconostoc mesenteroides) against causal agents of apple fruit rot, was investigated. Isolates of Colletotrichum acutatum, Colletotrichum gloeosporioides and Fusarium avenaceum were obtained from apple fruit samples expressing rot symptoms. Apple samples were collected during 2012 from Ultra Low Oxygen storages in Vojvodina Province, Serbia. The test microorganisms were identified according to pathogenic, morphological and ecological characteristics, and the identification of Colletotrichum isolates was confirmed by polimerase chain reaction (PCR). Antifungal effect was tested using wells method and the obtained data were processed by factorial ANOVA. Duncan's multiple range test was used to test significance of differences (p lt = 0.05) between mean values of measured radius of inhibition zones. Activity of S. cervisae, B. cereus and L. mesenteroides was not satisfactory. On the other hand, S. hygroscopicus expressed the strongest antagonism against all tested fungal isolates. The studied isolate of S. hygroscopicus showed significant antagonistic properties against storage pathogens of apple fruits identifying itself as promising biocontrol agent for commercial use. T2 - Romanian Biotechnological Letters T1 - Screening of antagonistic activity of selected microorganisms against apple rot pathogens EP - 8965 IS - 1 SP - 8959 VL - 19 UR - conv_650 ER -
@article{ author = "Grahovac, Mila and Balaž, Jelica and Grahovac, Jovana and Dodić, Jelena M. and Tanović, Brankica and Hrustić, Jovana and Tadijan, Ivana Z.", year = "2014", abstract = "Biological control of plant pathogens is considered an attractive alternative to chemical-based treatments because it has a minimal impact on the environment. In this study, in vitro antifungal efficiency of four microorganisms (Streptomyces hygroscopicus, Saccharomyces cervisae, Bacillus cereus and Leuconostoc mesenteroides) against causal agents of apple fruit rot, was investigated. Isolates of Colletotrichum acutatum, Colletotrichum gloeosporioides and Fusarium avenaceum were obtained from apple fruit samples expressing rot symptoms. Apple samples were collected during 2012 from Ultra Low Oxygen storages in Vojvodina Province, Serbia. The test microorganisms were identified according to pathogenic, morphological and ecological characteristics, and the identification of Colletotrichum isolates was confirmed by polimerase chain reaction (PCR). Antifungal effect was tested using wells method and the obtained data were processed by factorial ANOVA. Duncan's multiple range test was used to test significance of differences (p lt = 0.05) between mean values of measured radius of inhibition zones. Activity of S. cervisae, B. cereus and L. mesenteroides was not satisfactory. On the other hand, S. hygroscopicus expressed the strongest antagonism against all tested fungal isolates. The studied isolate of S. hygroscopicus showed significant antagonistic properties against storage pathogens of apple fruits identifying itself as promising biocontrol agent for commercial use.", journal = "Romanian Biotechnological Letters", title = "Screening of antagonistic activity of selected microorganisms against apple rot pathogens", pages = "8965-8959", number = "1", volume = "19", url = "conv_650" }
Grahovac, M., Balaž, J., Grahovac, J., Dodić, J. M., Tanović, B., Hrustić, J.,& Tadijan, I. Z.. (2014). Screening of antagonistic activity of selected microorganisms against apple rot pathogens. in Romanian Biotechnological Letters, 19(1), 8959-8965. conv_650
Grahovac M, Balaž J, Grahovac J, Dodić JM, Tanović B, Hrustić J, Tadijan IZ. Screening of antagonistic activity of selected microorganisms against apple rot pathogens. in Romanian Biotechnological Letters. 2014;19(1):8959-8965. conv_650 .
Grahovac, Mila, Balaž, Jelica, Grahovac, Jovana, Dodić, Jelena M., Tanović, Brankica, Hrustić, Jovana, Tadijan, Ivana Z., "Screening of antagonistic activity of selected microorganisms against apple rot pathogens" in Romanian Biotechnological Letters, 19, no. 1 (2014):8959-8965, conv_650 .