Differentiation of 'Candidatus Phytoplasma cynodontis' Based on 16S rRNA and groEL Genes and Identification of a New Subgroup, 16SrXIV-C
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2015
Authors
Mitrović, Jelena![](/themes/Mirageripest/images/orcid.png)
Smiljković, M.
Seemueller, Erich
Reinhardt, Richard
![](/themes/Mirageripest/images/orcid.png)
Huettel, Bruno
Buettner, Carmen
Bertaccini, Assunta
![](/themes/Mirageripest/images/orcid.png)
Kube, Michael
Duduk, Bojan
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Article (Published version)
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'Candidatus Phytoplasma cynodontis' is widespread in bermudagrass and has only been found in monocotyledonous plants. Molecular studies carried out on strains collected in Italy, Serbia, and Albania enabled verification of molecular variability in the 16S ribosomal RNA (rRNA) gene. Based on restriction fragment length polymorphism and sequence analyses, the strains from Serbia were clearly differentiated from all others and assigned to a new ribosomal DNA (rDNA) subgroup designated as 16SrXIV-C. A system for amplification of fragments containing the 'Ca. P. cynodontis' groEL gene was developed to enable study of its variability in related strains belonging to different 16SrXIV subgroups. Despite the fact that the groEL gene exhibited a greater sequence variation than 16S rRNA, the phylogenetic tree based on groEL gene sequence analysis was highly congruent with the 16S rDNA-based tree. The groEL gene analyses supported differentiation of the Serbian strains and definition of the new su...bgroup 16SrXIV-C. Phylogenetic analyses of both genes confirmed distinct phylogenetic lineages for strains belonging to 16SrXIV subgroups. Furthermore, groEL is the only nonribosomal marker developed for characterization of 'Ca. P. cynodontis' thus far, and its application in molecular surveys should provide better insight into the relationships among these phytoplasmas and correlation between strain differentiation and their geographical distribution.
Source:
Plant Disease, 2015, 99, 11, 1578-1583Publisher:
- AMER PHYTOPATHOLOGICAL SOC, ST PAUL
Projects:
- German Research Foundation (DFG) [KU 2679/2-1, BU 890/21-1]
- Max-Planck Society
- German Academic Exchange Service (DAAD) [56266384]
- Studies on plant pathogens, arthropods, weeds, and pesticides with a view to developing the methods of biorational plant protection and safe food production (RS-MESTD-Technological Development (TD or TR)-31043)
- 451-02-00446/2013-09/11
DOI: 10.1094/PDIS-01-15-0061-RE
ISSN: 0191-2917
PubMed: 30695967
WoS: 000364354300015
Scopus: 2-s2.0-84946592761
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Institution
Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Mitrović, Jelena AU - Smiljković, M. AU - Seemueller, Erich AU - Reinhardt, Richard AU - Huettel, Bruno AU - Buettner, Carmen AU - Bertaccini, Assunta AU - Kube, Michael AU - Duduk, Bojan PY - 2015 UR - https://ripest.pesting.org.rs/handle/123456789/327 AB - 'Candidatus Phytoplasma cynodontis' is widespread in bermudagrass and has only been found in monocotyledonous plants. Molecular studies carried out on strains collected in Italy, Serbia, and Albania enabled verification of molecular variability in the 16S ribosomal RNA (rRNA) gene. Based on restriction fragment length polymorphism and sequence analyses, the strains from Serbia were clearly differentiated from all others and assigned to a new ribosomal DNA (rDNA) subgroup designated as 16SrXIV-C. A system for amplification of fragments containing the 'Ca. P. cynodontis' groEL gene was developed to enable study of its variability in related strains belonging to different 16SrXIV subgroups. Despite the fact that the groEL gene exhibited a greater sequence variation than 16S rRNA, the phylogenetic tree based on groEL gene sequence analysis was highly congruent with the 16S rDNA-based tree. The groEL gene analyses supported differentiation of the Serbian strains and definition of the new subgroup 16SrXIV-C. Phylogenetic analyses of both genes confirmed distinct phylogenetic lineages for strains belonging to 16SrXIV subgroups. Furthermore, groEL is the only nonribosomal marker developed for characterization of 'Ca. P. cynodontis' thus far, and its application in molecular surveys should provide better insight into the relationships among these phytoplasmas and correlation between strain differentiation and their geographical distribution. PB - AMER PHYTOPATHOLOGICAL SOC, ST PAUL T2 - Plant Disease T1 - Differentiation of 'Candidatus Phytoplasma cynodontis' Based on 16S rRNA and groEL Genes and Identification of a New Subgroup, 16SrXIV-C EP - 1583 IS - 11 SP - 1578 VL - 99 DO - 10.1094/PDIS-01-15-0061-RE UR - conv_460 ER -
@article{ author = "Mitrović, Jelena and Smiljković, M. and Seemueller, Erich and Reinhardt, Richard and Huettel, Bruno and Buettner, Carmen and Bertaccini, Assunta and Kube, Michael and Duduk, Bojan", year = "2015", abstract = "'Candidatus Phytoplasma cynodontis' is widespread in bermudagrass and has only been found in monocotyledonous plants. Molecular studies carried out on strains collected in Italy, Serbia, and Albania enabled verification of molecular variability in the 16S ribosomal RNA (rRNA) gene. Based on restriction fragment length polymorphism and sequence analyses, the strains from Serbia were clearly differentiated from all others and assigned to a new ribosomal DNA (rDNA) subgroup designated as 16SrXIV-C. A system for amplification of fragments containing the 'Ca. P. cynodontis' groEL gene was developed to enable study of its variability in related strains belonging to different 16SrXIV subgroups. Despite the fact that the groEL gene exhibited a greater sequence variation than 16S rRNA, the phylogenetic tree based on groEL gene sequence analysis was highly congruent with the 16S rDNA-based tree. The groEL gene analyses supported differentiation of the Serbian strains and definition of the new subgroup 16SrXIV-C. Phylogenetic analyses of both genes confirmed distinct phylogenetic lineages for strains belonging to 16SrXIV subgroups. Furthermore, groEL is the only nonribosomal marker developed for characterization of 'Ca. P. cynodontis' thus far, and its application in molecular surveys should provide better insight into the relationships among these phytoplasmas and correlation between strain differentiation and their geographical distribution.", publisher = "AMER PHYTOPATHOLOGICAL SOC, ST PAUL", journal = "Plant Disease", title = "Differentiation of 'Candidatus Phytoplasma cynodontis' Based on 16S rRNA and groEL Genes and Identification of a New Subgroup, 16SrXIV-C", pages = "1583-1578", number = "11", volume = "99", doi = "10.1094/PDIS-01-15-0061-RE", url = "conv_460" }
Mitrović, J., Smiljković, M., Seemueller, E., Reinhardt, R., Huettel, B., Buettner, C., Bertaccini, A., Kube, M.,& Duduk, B.. (2015). Differentiation of 'Candidatus Phytoplasma cynodontis' Based on 16S rRNA and groEL Genes and Identification of a New Subgroup, 16SrXIV-C. in Plant Disease AMER PHYTOPATHOLOGICAL SOC, ST PAUL., 99(11), 1578-1583. https://doi.org/10.1094/PDIS-01-15-0061-RE conv_460
Mitrović J, Smiljković M, Seemueller E, Reinhardt R, Huettel B, Buettner C, Bertaccini A, Kube M, Duduk B. Differentiation of 'Candidatus Phytoplasma cynodontis' Based on 16S rRNA and groEL Genes and Identification of a New Subgroup, 16SrXIV-C. in Plant Disease. 2015;99(11):1578-1583. doi:10.1094/PDIS-01-15-0061-RE conv_460 .
Mitrović, Jelena, Smiljković, M., Seemueller, Erich, Reinhardt, Richard, Huettel, Bruno, Buettner, Carmen, Bertaccini, Assunta, Kube, Michael, Duduk, Bojan, "Differentiation of 'Candidatus Phytoplasma cynodontis' Based on 16S rRNA and groEL Genes and Identification of a New Subgroup, 16SrXIV-C" in Plant Disease, 99, no. 11 (2015):1578-1583, https://doi.org/10.1094/PDIS-01-15-0061-RE ., conv_460 .