First Report of 'Candidatus phytoplasma solani' in Sunflower in Bulgaria
Apstrakt
The molecular identification of phytoplasma from infected sunflower in Bulgaria is reported. During a survey in the Boliarsko area in the Yambol region of Bulgaria, phytoplasma-like symptoms were observed on sunflower. The observed symptoms included yellowing, deformations and proliferation of flowers and sterile (empty) seeds. The Polymerase Chain Reaction (PCR) analyses with primers specific for the 16S ribosomal gene of phytoplasmas confirmed that symptomatic samples of sunflower were infected by phytoplasmas. Phytoplasmas were identified using sequence analyses of PCR amplified 16S rDNA. The obtained sequence showed identity with the 'Candidatus Phytoplasma solani' strain from corn from Bulgaria and other 39 strains deposited in the GenBank. To our knowledge this is the first report of stolbur phytoplasma in sunflower (Helianthus annuus) in Bulgaria, adding a new cultivated plant-species to the already wide natural host range of stolbur phytoplasma.
Izvor:
Mitteilungen Klosterneuburg, 2016, 66, 1, 19-21Izdavač:
- HOHERE BUNDESLEHRANSTALT & BUNDESAMT WEIN-& OBSTBAU KLOSTERNEUBURG, KLOSTERNEUBURG
Institucija
Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Avramov, Zhelyu AU - Stepanović, Jelena AU - Panajotova, Dora AU - Laginova, Mariana AU - Duduk, Bojan PY - 2016 UR - https://ripest.pesting.org.rs/handle/123456789/362 AB - The molecular identification of phytoplasma from infected sunflower in Bulgaria is reported. During a survey in the Boliarsko area in the Yambol region of Bulgaria, phytoplasma-like symptoms were observed on sunflower. The observed symptoms included yellowing, deformations and proliferation of flowers and sterile (empty) seeds. The Polymerase Chain Reaction (PCR) analyses with primers specific for the 16S ribosomal gene of phytoplasmas confirmed that symptomatic samples of sunflower were infected by phytoplasmas. Phytoplasmas were identified using sequence analyses of PCR amplified 16S rDNA. The obtained sequence showed identity with the 'Candidatus Phytoplasma solani' strain from corn from Bulgaria and other 39 strains deposited in the GenBank. To our knowledge this is the first report of stolbur phytoplasma in sunflower (Helianthus annuus) in Bulgaria, adding a new cultivated plant-species to the already wide natural host range of stolbur phytoplasma. PB - HOHERE BUNDESLEHRANSTALT & BUNDESAMT WEIN-& OBSTBAU KLOSTERNEUBURG, KLOSTERNEUBURG T2 - Mitteilungen Klosterneuburg T1 - First Report of 'Candidatus phytoplasma solani' in Sunflower in Bulgaria EP - 21 IS - 1 SP - 19 VL - 66 UR - conv_471 ER -
@article{ author = "Avramov, Zhelyu and Stepanović, Jelena and Panajotova, Dora and Laginova, Mariana and Duduk, Bojan", year = "2016", abstract = "The molecular identification of phytoplasma from infected sunflower in Bulgaria is reported. During a survey in the Boliarsko area in the Yambol region of Bulgaria, phytoplasma-like symptoms were observed on sunflower. The observed symptoms included yellowing, deformations and proliferation of flowers and sterile (empty) seeds. The Polymerase Chain Reaction (PCR) analyses with primers specific for the 16S ribosomal gene of phytoplasmas confirmed that symptomatic samples of sunflower were infected by phytoplasmas. Phytoplasmas were identified using sequence analyses of PCR amplified 16S rDNA. The obtained sequence showed identity with the 'Candidatus Phytoplasma solani' strain from corn from Bulgaria and other 39 strains deposited in the GenBank. To our knowledge this is the first report of stolbur phytoplasma in sunflower (Helianthus annuus) in Bulgaria, adding a new cultivated plant-species to the already wide natural host range of stolbur phytoplasma.", publisher = "HOHERE BUNDESLEHRANSTALT & BUNDESAMT WEIN-& OBSTBAU KLOSTERNEUBURG, KLOSTERNEUBURG", journal = "Mitteilungen Klosterneuburg", title = "First Report of 'Candidatus phytoplasma solani' in Sunflower in Bulgaria", pages = "21-19", number = "1", volume = "66", url = "conv_471" }
Avramov, Z., Stepanović, J., Panajotova, D., Laginova, M.,& Duduk, B.. (2016). First Report of 'Candidatus phytoplasma solani' in Sunflower in Bulgaria. in Mitteilungen Klosterneuburg HOHERE BUNDESLEHRANSTALT & BUNDESAMT WEIN-& OBSTBAU KLOSTERNEUBURG, KLOSTERNEUBURG., 66(1), 19-21. conv_471
Avramov Z, Stepanović J, Panajotova D, Laginova M, Duduk B. First Report of 'Candidatus phytoplasma solani' in Sunflower in Bulgaria. in Mitteilungen Klosterneuburg. 2016;66(1):19-21. conv_471 .
Avramov, Zhelyu, Stepanović, Jelena, Panajotova, Dora, Laginova, Mariana, Duduk, Bojan, "First Report of 'Candidatus phytoplasma solani' in Sunflower in Bulgaria" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):19-21, conv_471 .