Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
2017
Преузимање 🢃
Аутори
Obradović, AnaStanković, Slavica
Krnjaja, Vesna
Nikolić, Ana
Ignjatovic-Micić, Dragana
Stepanović, Jelena
Duduk, Bojan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respecti...vely. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
Кључне речи:
trichothecene chemotype / NIV / Fusarium graminearum / 3ADON / 15-ADONИзвор:
Genetika-Belgrade, 2017, 49, 1, 355-364Издавач:
- Društvo genetičara Srbije, Beograd
Пројекти:
- Редукција токсигених гљива рода Фусариум и њихових микотоксина у производњи здравствено безбедне хране на бази жита (RS-MESTD-Technological Development (TD or TR)-31023)
DOI: 10.2298/GENSR1701355O
ISSN: 0534-0012
WoS: 000405684200030
Scopus: 2-s2.0-85026236193
Институција
Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Obradović, Ana AU - Stanković, Slavica AU - Krnjaja, Vesna AU - Nikolić, Ana AU - Ignjatovic-Micić, Dragana AU - Stepanović, Jelena AU - Duduk, Bojan PY - 2017 UR - https://ripest.pesting.org.rs/handle/123456789/395 AB - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions. PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia EP - 364 IS - 1 SP - 355 VL - 49 DO - 10.2298/GENSR1701355O UR - conv_493 ER -
@article{ author = "Obradović, Ana and Stanković, Slavica and Krnjaja, Vesna and Nikolić, Ana and Ignjatovic-Micić, Dragana and Stepanović, Jelena and Duduk, Bojan", year = "2017", abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia", pages = "364-355", number = "1", volume = "49", doi = "10.2298/GENSR1701355O", url = "conv_493" }
Obradović, A., Stanković, S., Krnjaja, V., Nikolić, A., Ignjatovic-Micić, D., Stepanović, J.,& Duduk, B.. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 49(1), 355-364. https://doi.org/10.2298/GENSR1701355O conv_493
Obradović A, Stanković S, Krnjaja V, Nikolić A, Ignjatovic-Micić D, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika-Belgrade. 2017;49(1):355-364. doi:10.2298/GENSR1701355O conv_493 .
Obradović, Ana, Stanković, Slavica, Krnjaja, Vesna, Nikolić, Ana, Ignjatovic-Micić, Dragana, Stepanović, Jelena, Duduk, Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" in Genetika-Belgrade, 49, no. 1 (2017):355-364, https://doi.org/10.2298/GENSR1701355O ., conv_493 .