Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw
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2019
Authors
Grujić, MaricaDojnov, Biljana
Potočnik, Ivana
Atanasova, Lea
Duduk, Bojan
Srebotnik, Ewald
Druzhinina, Irina S.
Kubicek, Christian P.
Vujcić, Zoran
Article (Published version)
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Lignocellulosic plant biomass is the world's most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain-UB483FTG2/TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the ...model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (beta-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.
Keywords:
Wheat straw / Trichoderma / Trichoderma reesei / Trichoderma guizhouense / Cellulases / Cellulase enzyme complexSource:
World Journal of Microbiology & Biotechnology, 2019, 35, 12Publisher:
- SPRINGER, DORDRECHT
Projects:
- Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection (RS-MESTD-Basic Research (BR or ON)-172048)
- Austrian Science Fund (FWF) [P 25613 B20]
DOI: 10.1007/s11274-019-2774-y
ISSN: 0959-3993
PubMed: 31776792
WoS: 000517949400001
Scopus: 2-s2.0-85075668082
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Institution
Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Grujić, Marica AU - Dojnov, Biljana AU - Potočnik, Ivana AU - Atanasova, Lea AU - Duduk, Bojan AU - Srebotnik, Ewald AU - Druzhinina, Irina S. AU - Kubicek, Christian P. AU - Vujcić, Zoran PY - 2019 UR - https://ripest.pesting.org.rs/handle/123456789/474 AB - Lignocellulosic plant biomass is the world's most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain-UB483FTG2/TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (beta-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes. PB - SPRINGER, DORDRECHT T2 - World Journal of Microbiology & Biotechnology T1 - Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw IS - 12 VL - 35 DO - 10.1007/s11274-019-2774-y UR - conv_536 ER -
@article{ author = "Grujić, Marica and Dojnov, Biljana and Potočnik, Ivana and Atanasova, Lea and Duduk, Bojan and Srebotnik, Ewald and Druzhinina, Irina S. and Kubicek, Christian P. and Vujcić, Zoran", year = "2019", abstract = "Lignocellulosic plant biomass is the world's most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain-UB483FTG2/TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (beta-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.", publisher = "SPRINGER, DORDRECHT", journal = "World Journal of Microbiology & Biotechnology", title = "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw", number = "12", volume = "35", doi = "10.1007/s11274-019-2774-y", url = "conv_536" }
Grujić, M., Dojnov, B., Potočnik, I., Atanasova, L., Duduk, B., Srebotnik, E., Druzhinina, I. S., Kubicek, C. P.,& Vujcić, Z.. (2019). Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology & Biotechnology SPRINGER, DORDRECHT., 35(12). https://doi.org/10.1007/s11274-019-2774-y conv_536
Grujić M, Dojnov B, Potočnik I, Atanasova L, Duduk B, Srebotnik E, Druzhinina IS, Kubicek CP, Vujcić Z. Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology & Biotechnology. 2019;35(12). doi:10.1007/s11274-019-2774-y conv_536 .
Grujić, Marica, Dojnov, Biljana, Potočnik, Ivana, Atanasova, Lea, Duduk, Bojan, Srebotnik, Ewald, Druzhinina, Irina S., Kubicek, Christian P., Vujcić, Zoran, "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw" in World Journal of Microbiology & Biotechnology, 35, no. 12 (2019), https://doi.org/10.1007/s11274-019-2774-y ., conv_536 .