Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis
2021
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Аутори
Zambounis, AntoniosStefanidou, Eleni
Madesis, Panagiotis
Hrustić, Jovana
Mihajlović, Milica
Tanović, Brankica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Monilinia laxa, Monilinia fructicola and Monilinia fructigena are the three main causal agents of brown rot, which is one of the most important diseases of stone fruits in pre- and postharvest conditions. Nowadays, the need for the precise genotyping of these Monilinia species in terms of the genetic diversity of their populations or differences in their pathogenicity and host range is a prerequisite for any efficient disease management. In our study, the genetic structure of Monilinia populations in Serbia from three geographically distinct regions was investigated employing a high-resolution melting (HRM) analysis which is a sensitive and rapid molecular approach in fungal genotyping and diagnostics. Using species-specific primer pairs genotype-specific HRM melting curve profiles were generated allowing to efficiently decipher the genetic diversity of the Monilinia populations. The Monilinia genotypes could be easily distinguished according to their melting curves. The isolates from ...the northern region were assigned to distinct genotypes and grouped rather independently compared to the isolates of the other two regions among all three tested Monilinia spp. M. fructicola and M. fructigena showed a higher genetic diversity among their populations (44%) compared with the genetic diversity among the M. laxa populations (7%). In contrast, the genetic variance within the pathogen populations was higher in the case of M. laxa (93%). Our data revealed an absence of host specificity in the Monilinia spp. populations.
Кључне речи:
population genotyping / brown rotИзвор:
Plant Protection Science, 2021, 57, 1, 38-46Издавач:
- Prague : Czech Academy of Agricultural Sciences
Пројекти:
- Hellenic Agricultural Organization (HAO) -Demeter of Greece
- Ministry of Education and Science of the Republic of Serbia
DOI: 10.17221/35/2020-PPS
ISSN: 1212-2580
WoS: 000597397000005
Scopus: 2-s2.0-85097910045
Институција
Institut za pesticide i zaštitu životne sredineTY - JOUR AU - Zambounis, Antonios AU - Stefanidou, Eleni AU - Madesis, Panagiotis AU - Hrustić, Jovana AU - Mihajlović, Milica AU - Tanović, Brankica PY - 2021 UR - https://ripest.pesting.org.rs/handle/123456789/525 AB - Monilinia laxa, Monilinia fructicola and Monilinia fructigena are the three main causal agents of brown rot, which is one of the most important diseases of stone fruits in pre- and postharvest conditions. Nowadays, the need for the precise genotyping of these Monilinia species in terms of the genetic diversity of their populations or differences in their pathogenicity and host range is a prerequisite for any efficient disease management. In our study, the genetic structure of Monilinia populations in Serbia from three geographically distinct regions was investigated employing a high-resolution melting (HRM) analysis which is a sensitive and rapid molecular approach in fungal genotyping and diagnostics. Using species-specific primer pairs genotype-specific HRM melting curve profiles were generated allowing to efficiently decipher the genetic diversity of the Monilinia populations. The Monilinia genotypes could be easily distinguished according to their melting curves. The isolates from the northern region were assigned to distinct genotypes and grouped rather independently compared to the isolates of the other two regions among all three tested Monilinia spp. M. fructicola and M. fructigena showed a higher genetic diversity among their populations (44%) compared with the genetic diversity among the M. laxa populations (7%). In contrast, the genetic variance within the pathogen populations was higher in the case of M. laxa (93%). Our data revealed an absence of host specificity in the Monilinia spp. populations. PB - Prague : Czech Academy of Agricultural Sciences T2 - Plant Protection Science T1 - Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis EP - 46 IS - 1 SP - 38 VL - 57 DO - 10.17221/35/2020-PPS UR - conv_551 ER -
@article{ author = "Zambounis, Antonios and Stefanidou, Eleni and Madesis, Panagiotis and Hrustić, Jovana and Mihajlović, Milica and Tanović, Brankica", year = "2021", abstract = "Monilinia laxa, Monilinia fructicola and Monilinia fructigena are the three main causal agents of brown rot, which is one of the most important diseases of stone fruits in pre- and postharvest conditions. Nowadays, the need for the precise genotyping of these Monilinia species in terms of the genetic diversity of their populations or differences in their pathogenicity and host range is a prerequisite for any efficient disease management. In our study, the genetic structure of Monilinia populations in Serbia from three geographically distinct regions was investigated employing a high-resolution melting (HRM) analysis which is a sensitive and rapid molecular approach in fungal genotyping and diagnostics. Using species-specific primer pairs genotype-specific HRM melting curve profiles were generated allowing to efficiently decipher the genetic diversity of the Monilinia populations. The Monilinia genotypes could be easily distinguished according to their melting curves. The isolates from the northern region were assigned to distinct genotypes and grouped rather independently compared to the isolates of the other two regions among all three tested Monilinia spp. M. fructicola and M. fructigena showed a higher genetic diversity among their populations (44%) compared with the genetic diversity among the M. laxa populations (7%). In contrast, the genetic variance within the pathogen populations was higher in the case of M. laxa (93%). Our data revealed an absence of host specificity in the Monilinia spp. populations.", publisher = "Prague : Czech Academy of Agricultural Sciences", journal = "Plant Protection Science", title = "Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis", pages = "46-38", number = "1", volume = "57", doi = "10.17221/35/2020-PPS", url = "conv_551" }
Zambounis, A., Stefanidou, E., Madesis, P., Hrustić, J., Mihajlović, M.,& Tanović, B.. (2021). Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis. in Plant Protection Science Prague : Czech Academy of Agricultural Sciences., 57(1), 38-46. https://doi.org/10.17221/35/2020-PPS conv_551
Zambounis A, Stefanidou E, Madesis P, Hrustić J, Mihajlović M, Tanović B. Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis. in Plant Protection Science. 2021;57(1):38-46. doi:10.17221/35/2020-PPS conv_551 .
Zambounis, Antonios, Stefanidou, Eleni, Madesis, Panagiotis, Hrustić, Jovana, Mihajlović, Milica, Tanović, Brankica, "Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis" in Plant Protection Science, 57, no. 1 (2021):38-46, https://doi.org/10.17221/35/2020-PPS ., conv_551 .